Hi,

I'm using CompassXport (Free download from Bruker, after
registration), not CompassXtract

>compassxport -multi d:\data\directory\

will convert all *.yep files of the given data directory to *.mzXML
files which can be read by TPP/X!Tandem. It will automatically dive
into your .D directories.

I used this tool successfully for converting data of the HCTultra/ETD
(Bruker) and the MSD/XCTultra (Agilent).

Those *.mzXML files work only for X!Tandem, not for OMSSA.

For generating *.mgf files usable for OMSSA searches I use
MzXML2Search, bundled with the TPP:

>FOR %I IN (*.mzXML) DO MzXML2Search -mgf -ACID -M2-2 -P5 -I0.01 -B10 -T5000.0 
>%I

converts all *.mzXML files to OMSSA compatible *.mgf

Saludos, Robert


On Feb 11, 1:04 pm, Matt Chambers <[email protected]>
wrote:
> As Natalie suggested, Analysis.yep indicates the Bruker/Agilent YEP
> format which ProteoWizard reads with CompassXtract. Msconvert will
> automatically detect the format of the .d directory and use the
> appropriate reader. Keep in mind the CompassXtract interface (which
> supports Bruker FID/BAF and Bruker/Agilent YEP) is only licensed for
> non-commercial use.
>
> -Matt
>
> Natalie Tasman wrote:
> > Hello Zeyu,
>
> > You're using the TPP's "trapper" program.  Trapper is designed for
> > data from Agilent's *MassHunter* program.  This data is stored in a .d
> > folder.  However, there is a confusing situation: two different
> > Agilent formats use .d folders.  Your .d folder has the different
> > format: analysis.yep.  I'm guessing that you're probably using data
> > acquired with Agilent's *ChemStation* program.  Trapper does not
> > handle .yep.  But, msconvert, which is included with the TPP and comes
> > from the ProteoWizard (pwiz) project, might handle this type of data.  
> > I'm forwarding this message to the pwiz discussion list as well, and
> > hopefully they can suggest a way to proceed.
>
> > There is an
>
> > -Natalie
>
> > On 2/10/10 11:16 PM, zeyu sun wrote:
> >> Hi, I have a problem to convert the .d folder from a ETD trap to xml 
> >> format. I have no problem to convert .d folderto xml for spectrum from 
> >> Q-tof. Here is what I saw in the commend line:
>
> >> Settings:
> >>   centroid scans: 0
>
> >>   compress scans: 0
> >>   verbose mode: 1
> >>   skip checksum: 0
> >>   deisotope:0
> >>   requirePeptideLikeAbundanceProfile: 0
> >>   relativeTolerance: 0
> >>   absoluteTolerance: 0
> >>   limitChargeState:0
> >>   maxChargeState: 0
>
> >>   mzML mode: 0
> >>   mzXML mode: 1
> >>   input filename: c:/Inetpub/wwwroot/ISB/data/ZSMOAC.D
> >>   output filename: c:/Inetpub/wwwroot/ISB/data/ZSMOAC.mzXML
>
> >> ERROR at .\MHDACWrapper.cpp, 60:
> >> HR = -2147024894
> >> OpenDataFile
>
> >> unable to open c:/Inetpub/wwwroot/ISB/data/ZSMOAC.D with MassHunter 
> >> interface
>
> >> *Command FAILED*
>
> >> by the way, the .d folder comprised of a analysis.yep file (very big,
> >> which I assume is where the MS data stored), a LCDIAG.reg file, a
> >> extension.baf file, a sample.mac file, 3 txt files named
> >> LCMETHODAUDITTRAIL, lcparms, RUN, and a folder ETD_LONGCHIP_100128.M.
>
> >> Any thought how to do the conversion?
> >> Thank you very much!
>
> >> --
> >> Zeyu Sun
>
>

-- 
You received this message because you are subscribed to the Google Groups 
"spctools-discuss" group.
To post to this group, send email to [email protected].
To unsubscribe from this group, send email to 
[email protected].
For more options, visit this group at 
http://groups.google.com/group/spctools-discuss?hl=en.

Reply via email to