Hi Zhang, As Jimmy pointed out, identification from MS1 level data is too general to be considered conclusive evidence, although it provides a fast way of identifying (potential) peptides. The amount of information in MS1 is too low to draw conclusions. MS2 has peptide fragments (as opposed to protein fragments or peptides at MS1 level) and the info from the peptide fragments (consider them as amino acids-for details refer to ABCc and XYZs of peptide sequencing as mentioned by Jimmy) can help decipher the sequence.
Finally a lot of tools are available to do both MS1 and MS2 level interpretations. You may try http://www.matrixscience.com/cgi/search_form.pl?FORMVER=2&SEARCH=PMF for your purpose. <http://www.matrixscience.com/cgi/search_form.pl?FORMVER=2&SEARCH=PMF> Regards, Amit Kumar Yadav Senior Research Fellow (SRF-CSIR) IGIB, New Delhi (India) http://masswiz.igib.res.in On Thu, Mar 4, 2010 at 7:09 AM, Jimmy Eng <[email protected]> wrote: > Look up accurate mass and time tags, peptide mass fingerprinting, and > maybe even a general proteomics data analysis review such as doi: > 10.1038/nrm1468 > > I hope you don't take this too negatively but the scope of what one > might consider ms1 data is too general (a peptide mass fingerprint > spectra, and entire LC/MS run, etc.) and the questions you're asking > implies that you need more education on the topic before even > considering doing data analysis. > > On Feb 24, 7:40 am, Fan Zhang <[email protected]> wrote: > > Hi There, > > > > I have only Ms level 1 data. Can we identify peptide from ms1 data? > > > > If we can, which tool to use? > > > > Thanks. > > > > Van > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To post to this group, send email to [email protected]. > To unsubscribe from this group, send email to > [email protected]<spctools-discuss%[email protected]> > . > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. > > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
