Hi Darryl,

Thanks for your lead.

I could run the the search but could not view the search results in
bioworks. After converting the DTA and OUT files to SRF  I could open the
search results but found no hits listed eventhough some of the OUT files has
peptides identified

Then I brought the DTA and OUT (as well as SRF separately) files to scaffold
and Theres turned out to be no results either.
Any idea why it could happen??

Also what are the other tools for sequest search using MGF, DTA  etc.??

Thanks
Tamanna


On Wed, Mar 10, 2010 at 12:14 PM, Darryl Davis <[email protected]> wrote:

> For dta work try Actions- Sequest search.  Choose folders w/ DTAs deselect
> use SRF file.  Deselect dta generation.  Choose sequest.params file and then
> start search.
>
> If you have mgf or dta format already there are other tools than Bioworks
> to use (ofcourse I state the obvious).
> DD
>
>
> On Wed, Mar 10, 2010 at 11:54 AM, Tamanna Sultana <
> [email protected]> wrote:
>
>> Does anyone knows a way of importing MGF or DTA files to Bioworks for
>> a Sequest search?
>>
>> I have a dataset in *.mgf format and I would like to do a Sequest
>> search.
>>
>> 1. I could not import mgf to bioworks. I know that sequest uses *.dta
>> file for search, so I converted the mgf to dta but failed to import
>> the dta to bioworks. Bioworks seem to accept *.RAW only.
>>
>> 2. I saw under tools in 'Bioworks' I could import spectral data in
>> mzdata format, that did not work either.
>>
>> Anyone have any suggestion??
>> Thanks
>> Tamanna
>>
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-- 
Tamanna S Rahman

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