Florian, Looked at our MSE conversions using MassWolf and we don't have any lockspray data. The conversion ignores function 3 (lockspray) from the RAW data. The regular pattern still looks suspicially like lockspray though, maybe MassWolf not recongising the function as lockspray? I guess that if the spectra look like calibration compound that's what it is, otherwise I have no idea. What instrument / MassLynx version is it?
On 5 Apr, 17:50, Dave Trudgian <[email protected]> wrote: > Florian, > > Is this due to lockspray scans? If you look at the spectra of the > "extra" low energy scans, are they all very similar, i.e. showing the > calibration compound? The scan numbering looks a lot like it could be. I > don't have any masswolf conversions of our MSE data to hand, but I will > check tomorrow and let you know. > > DT > > On 05/04/2010 15:21, fmg85 wrote: > > > Dear developers, users and especially Rune :-) > > > Masswolf seems to have a problem with the low energy MSE data in MSE > > files. I should have 291 scans in both, the low and high energy scans > > (I checked this in Masslynx and also parsing the via Water's > > Databridge converted netCDF files). The high energy data has 291 > > scans, the low however changes to 365. > > If I look at the acquisitionNumbers then I can see that every other > > (5.) scan the low energy function "steels" a scan from the high energy > > function as you can clearly see here: > > low: 1 2 4 6 8 10 11 13 15 17 19 20 22 24 26 > > high: 3 5 7 9 12 14 16 18 21 23 25 27 > > You can also see that the file looks funny in the Insilicos viewer > > (random scan every other value) > > If I use waters Databridge to convert my MSE into two datafiles > > (netCDF), then convert them back into two waters .raw functions (high/ > > low energy file) and use masswolf to individually convert them into a > > mzXML function the scans are correct (both have 291 each) and look > > normal in the insilicos viewer. Centroiding does not have any > > influence on this. > > Does anyone have an idea what this could be caused? I verified this on > > different MSE files and all software is up2date. > > > Best wishes, > > > Florian -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
