Hi Simon,

This error indicates that the PepXMLViewer failed when it tried to
index your pepxml file.  .pepprophet.xml is not a normal extension
produced by the TPP for a pepXML file.  Can you describe exactly how
you arrived at this pepXML file?

Thanks,
-David

On Wed, Jun 9, 2010 at 1:46 AM, Simon Michnowicz
<[email protected]> wrote:
> Dear Group,
> I ran  xinteract on a pepXML file that was generated from a Mascot
> search, and it exited with an error message.
>
> command "/mnt/sanfs/APCF-PROD/APCF_WEB/tpp/cgi-bin/PepXMLViewer.cgi -
> I /mnt/sanfs/APCF-PROD/results/tpp/2010-06-08/simon-
> J2468.pepprophet.xml" exited with non-zero exit code: 11
>
> I am afraid I do not know what this error means. I found the input
> pepXML only had a few number of entries, so am I right to think that
> this is cause of the error?(The Mascot search was done using
> incorrect parameters, resulting in a poor output)
> I have two questions:
> (1) How much data do you need to provide a satisfactory run for
> peptideProphet? Is it possible to check this before calling xinteract?
> (2) I looked at the .dat=>pepXML conversion via Mascot2XML. I expected
> a 1:1 mapping for proteins, however I found only a subset of proteins
> being placed in the file. Is there some internal filtering that occurs
> here? The scores were all low, which might have taxed any algorithm to
> do this.
>
> Much thanks for any assistance.
> Simon Michnowicz
>
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