Dear Group I'm using peptideSieve v0.51 (windows version with GUI) in order to predict the likelyhood that some proteins of interest can be seen in a proteomic experiment. The problem is that some proteins are completely not present in the output even if teorically they could be cleaved by trypsin(I tryed with peptidecutter software). This in my opinion is strange, because for the peptides obtained by in silico digestion, I should obtain a value of their likelyhood... I'm not considering anything? Thank you very much for any help you could give to me
Below I paste one of the sequences for which I don't find any peptide in the output As parameters I select: all the experimental design, peptide length 3-50 missed cleavage 1 threshold score 0 MAQGTLIRVTPEQPTHAVCVLGTLTQLDICSSAPEDCTSFSINASPGVVVDIAHGPPAKK KSTGSSTWPLDPGVEVTLTMKVASGSTGDQKVQISYYGPKTPPVKALLYLTGVDGVSPCH PGWSAMA -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
