Hi everyone, I am just a beginner in MRM and my first step end up in mud. I am using TPP ver4.4.1 vuvuzela and downloaded the 18 protein MaRiMba tutorial files. I followed the instructions in the pdf files attached with the tutorial files, then I saw the red "FAILED". According to the log message, there is an error 9 with filterMRM. I did monor change to the criteria used for filter and still could not get my first MRM list.
I attach the log message here and hope for your advices. Thank you all! Run MaRiMba started at: Fri Apr 29 21:09:22 2011 ...Refreshing library against database ...Filtering out non-proteotypic and unmapped peptides SpectraST started at Fri Apr 29 21:09:23 2011. Creating library from "c:/Inetpub/wwwroot/ISB/data/MaRiMba/ 18mix_Mix7_Raw.splib" REFRESHING protein mappings...DONE! Importing peptide ions...500...1000...DONE! Library file (BINARY) "tmp_refreshed.splib" created. Library file (TEXT) "tmp_refreshed.sptxt" created. M/Z Index file "tmp_refreshed.spidx" created. Peptide Index file "tmp_refreshed.pepidx" created. Total number of spectra in library: 11679 Total number of distinct peptide ions in library: 1107 Total number of distinct stripped peptides in library: 811 CHARGE +1: 0 ; +2: 3742 ; +3: 4512 ; +4: 2591 ; +5: 834 ; > +5: 0 TERMINI Tryptic: 10334 ; Semi-tryptic: 1333 ; Non-tryptic: 12 PROBABILITY >0.9999: 5618 ; 0.999-0.9999: 2753 ; 0.99-0.999: 1875 ; 0.9-0.99: 1432 <0.9: 1 ...skipping 76 lines of output... Library file (BINARY) "tmp_MRM.splib" created. Library file (TEXT) "tmp_MRM.sptxt" created. M/Z Index file "tmp_MRM.spidx" created. Peptide Index file "tmp_MRM.pepidx" created. MRM Table file "tmp_MRM.mrm" created. Total number of spectra in library: 118 Total number of distinct peptide ions in library: 118 Total number of distinct stripped peptides in library: 88 CHARGE +1: 0 ; +2: 75 ; +3: 34 ; +4: 8 ; +5: 1 ; >+5: 0 TERMINI Tryptic: 118 ; Semi-tryptic: 0 ; Non-tryptic: 0 PROBABILITY >0.9999: 87 ; 0.999-0.9999: 15 ; 0.99-0.999: 11 ; 0.9-0.99: 5 <0.9: 0 NREPS 20+: 28 ; 10-19: 26 ; 4-9: 44 ; 2-3: 11 ; 1: 9 MODIFICATIONS C,Carbamidomethyl: 36 Total Run Time = 0 seconds. SpectraST finished at Fri Apr 29 21:12:16 2011 without error. ...Applying user-defined fiters ERROR: there was a problem with filterMRM: 9 Command FAILED -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
