Hi Kinfai,

The problem lies in the error message:
(mspire: ms-sequest
 not recognizedspire: ms-sequest)

We have not processed this sort of data yet.  If you want to post your
file here we can adjust the TPP to deal with these files in the
future:

http://tools.proteomecenter.org/wiki/index.php?title=TPP:Frequently_Asked_Questions#How_do_I_upload_files_to_the_SPC_tools_team.3F

Thanks,
-David



On Wed, Jun 22, 2011 at 12:00 AM, Kinfai Au <[email protected]> wrote:
> After converting SQT file to pep.xml file, I ran the following
> command:
>
>  xinteract  -Ninteract.pep.xml -p0.05 -l7 -O test5.pep.xml
>
> Then, it crashed in some step. Here is the error message. I spent days
> on it but the pipeline TPP still fails. Could anyone give me some
> help?
> ################################################################
>
> xinteract (TPP v4.4 VUVUZELA rev 1, Build 201106201323 (linux))
>
> running: "/home/stow/swtree/stow/TPP-4.4.1/bin/InteractParser
> 'interact.pep.xml' 'test5.pep.xml' -L'7'"
>  file 1: test5.pep.xml
>  processed altogether 41651 results
>
>
>  results written to file /home/kinfai/test5/interact.pep.shtml
>
>
>
> command completed in 44 sec
>
> running: "/home/stow/swtree/stow/TPP-4.4.1/bin/PeptideProphetParser
> 'interact.pep.xml' MINPROB=0.05"
> )(mspire: ms-sequest
>  not recognizedspire: ms-sequest
>
> command "/home/stow/swtree/stow/TPP-4.4.1/bin/PeptideProphetParser
> 'interact.pep.xml' MINPROB=0.05" exited with non-zero exit code: 256
> QUIT - the job is incomplete
>
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