Precursor charge is already in mzXML. If you look at the mzXML files created by ReAdW (msconvert should also have it), you should see lines such as: <precursorMz precursorIntensity="2123.5" precursorCharge="4" activationMethod="CID" >1390.82</precursorMz> Those lines are part of the msms scan information (ms level = 2). If the line isn't there, you might want to check the method on LTQ to make sure that Charge State Screening is enabled for the segment in the dependent scan settings.
Greg On Mon, Jul 11, 2011 at 11:06 AM, Robert <[email protected]>wrote: > Hi, > > when converting Thermo (LTQ velos) raw files to mzXML, no precursor > charges are included in the resulting mzXML. I tried ReAdW (TPP) and > msconvert (Proteowizzard). > > This is not a problem for X!Tandem searches, but for some uses > (calculation of mass errors, PRIDE XML repository), the precursor > charge would be desirable. > > Any suggestions, how the charge could be added to mzXML? > > Best, Robert > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To post to this group, send email to [email protected]. > To unsubscribe from this group, send email to > [email protected]. > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. > > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
