I also use AsapRatio and I frequently have NAs in my results and as you sad I am wondering of it is an experimental problem. Does anyone have experienced this problem before? What should I do to avoid it? regards, Julia
Em quinta-feira, 26 de julho de 2012 14h58min44s UTC-3, Daniel FS escreveu: > I'm using TPP v4.4 vuvuzela rev 1 and I'm working with SILAC cells. > Everything is going right until the quantification time, when my result > seems to be very bad. After 8 runs with different proportions of > heavy/light cells (1:1, 2:1) in a following conditions (Thermo Orbitrap -> > Mascot Search with Fixed Mod. on Carbamidomethyl and Variable Mod. on > Label(6)K and Oxidation(M) -> Analysis Pipeline with XPRESS mass diference > (K) 6.02 and ASAPRatio with change label K and Specific mass residue K > 134.114), my results are around 70% N_A, and just 20% really quantified. My > question is: why there is so much N_A in my results? I know that N_A is > something about cannot quantify, but what it is exactly? Is it something > experimental or is it a bad use of TPP? > > Thanks in advance, > > Daniel > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To view this discussion on the web visit https://groups.google.com/d/msg/spctools-discuss/-/__pLTZiDqV4J. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
