I've never gotten the static option to work. I'm not sure how the static option is intended to be used..... correctly e.g. What type of data set.
If you are doing two searches one with the static light and the second with the static heavy isotope labels, you will have to redo the search and do it as per the Tandem API where you set the light to be a static mod and the mass difference between the heavy and light to be a variable mod. In addition to giving results ASAPRatio can quantitate, it has been noted that the second search method is more statistically sound because it increases the size of the database as a result of the variable mods. Using the second search strategy has reduced ASAPRatio processing time on my data sets from many hours to about a half hour. Also, using the static option on Linux w/ TPP 4.5.2 ASAPRatio would complete after hours. But I have problems with the viewers not working in Linux so after switching to Win for the quan analysis, using TPP 4.6.1 the static option always kicks out an error and quits. Feeding it search results as mentioned above it all works and completes much more quickly. Why the static option doesn't work on our data sets is a mystery to me. Brian On Nov 21, 2012, at 8:33 AM, Alex <[email protected]> wrote: > I think my post didn't show up correctly. Here it goes again! > > Hi TTP community, > > I'm running some ASAPratio quant on a 2D-LC-MSMS experiment on windows XP > running TPP v4.5 RAPTURE rev 2, Build 201202031108 (MinGW). After a > couple of hours analysis I get the following error. Has somebody run into > this problem before??? Any ideas much appreciated!!! > > > Alex > > > Error Message: > > This application has requested the Runtime to terminate it in an unusual way. > Please contact the application's support team for more information. > command "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "interact.pep.xml" -lKn -F > -C -r0.1 -S -mK160.15137n33.06425" failed: No such process > command "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "interact.pep.xml" -lKn -F > -C -r0.1 -S -mK160.15137n33.06425" exited with non-zero exit code: 3 > QUIT - the job is incomplete > command "c:\Inetpub\tpp-bin\xinteract -Ninteract.pep.xml -p0.05 -l7 -Op -I1 > -I4 -I5 -A-lKn-F-C-r0.1-S-mK160.15137n33.06425 2DYeast01.tandem.pep.xml > 2DYeast02.tandem.pep.xml 2DYeast03.tandem.pep.xml 2DYeast04.tandem.pep.xml > 2DYeast05.tandem.pep.xml 2DYeast06.tandem.pep.xml > 2DYeast07.tandem.pep.xml 2DYeast08.tandem.pep.xml 2DYeast09.tandem.pep.xml > 2DYeast10.tandem.pep.xml 2DYeast11.tandem.pep.xml > 2DYeast12.tandem.pep.xml 2DYeast13.tandem.pep.xml 2DYeast14.tandem.pep.xml > 2DYeast15.tandem.pep.xml 2DYeast16.tandem.pep.xml > 2DYeast17.tandem.pep.xml 2DYeast18.tandem.pep.xml 2DYeast19.tandem.pep.xml > 2DYeast20.tandem.pep.xml 2DYeast21.tandem.pep.xml > 2DYeast22.tandem.pep.xml 2DYeast23.tandem.pep.xml H2DYeast01.tandem.pep.xml > H2DYeast02.tandem.pep.xml H2DYeast03.tandem.pep.xml > H2DYeast04.tandem.pep.xml H2DYeast05.tandem.pep.xml H2DYeast06.tandem.pep.xml > H2DYeast07.tandem.pep.xml H2DYeast08.tandem.pep.xml > H2DYeast09.tandem.pep.xml H2DYeast10.tandem.pep.xml H2DYeast11.tandem.pep.xml > H2DYeast12.tandem.pep.xml H2DYeast13.tandem.pep.xml > H2DYeast14.tandem.pep.xml H2DYeast15.tandem.pep.xml H2DYeast16.tandem.pep.xml > H2DYeast17.tandem.pep.xml H2DYeast18.tandem.pep.xml > H2DYeast19.tandem.pep.xml H2DYeast20.tandem.pep.xml H2DYeast21.tandem.pep.xml > H2DYeast22.tandem.pep.xml H2DYeast23.tandem.pep.xml" failed: No > such process > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To view this discussion on the web visit > https://groups.google.com/d/msg/spctools-discuss/-/K9ou37m9EhEJ. > To post to this group, send email to [email protected]. > To unsubscribe from this group, send email to > [email protected]. > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
