Hi Joe,

Thanks for your quick response. I have put all the remaining output files 
into dropbox now. Please take a look and let me know if you need any other 
information.

Vidya



On Friday, November 30, 2012 6:44:45 PM UTC-5, [email protected] 
wrote:
>
> Hi Vidya,
>
> Thanks for providing such great detail on the issue and I'm sorry it isn't 
> working for you.  We're taking a look at what you posted and there is an 
> error indicating invalid XML formatting in one of your files.  
> Unfortunately this is one of the ones that you haven't posted to dropbox.  
> Any chance you can upload the following file from your system (from the 
> "not working" case)?
>
> C:/Inetpub/wwwroot/ISB/data/data/interact.pep.xml"
>
> Thanks,
> Joe
>
> On Fri, Nov 30, 2012 at 3:04 PM, Vidya Venkatraman 
> <[email protected]<javascript:>
> > wrote:
>
>> Hi,
>>
>> I have encountered an issue wherein if I try to convert a mascot .dat 
>> file to pepxml, it does the conversion and when I try to use this pepxml 
>>  to run peptideprophet and proteinprophet, it fails. 
>>
>> I tried to check if .dat to pepxml conversion worked properly, but 
>> realised that when I click on the pepxml, nothing loads on the pepxml 
>> viewer and I also saw that usually there is an index file that is created 
>> and in this case, it did not create the index file as well. Just to make 
>> sure the .dat file is good, i was also able to open it up on Scaffold and 
>> see some proteins.
>>
>> This is the link to the shared dropbox folder called "TPP"
>> https://www.dropbox.com/sh/4unsley0gir7jve/7D3-XANnj8
>>
>> I have 2 folders there, one called "working files" where I tested a .dat 
>> file (from same samle, different fraction) conversion to pepxml and ran 
>> peptide and protein prophet and it worked successfully. The other folder 
>> called "not working files" has the .dat file which failed to run peptide 
>> and protein prophet. Also attached are .mzxml, .pepxml, the log of 
>> conversion from .dat to .pepxml and errorlog of conversion from pepxml to 
>> peptide and protein prophet along with screenshots. I have also included 
>> the fasta file I used in the "Fasta" folder. 
>>
>> Can you anyone guide me as to what could have gone wrong?
>>
>> Thanks in advance,
>> Vidya
>>
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