Hi all, I've been reading a lot lately about a variety of label-free quantitation techniques. Unfortunately our data is poorly suited to MS1 peak extraction/integration (a la maxquant, skyline MS1 filtering etc.) and so we're very keen to implement some form of spectral counting (MS2 level) to compare complex co-purification from two different conditions. It seems that in the world of spectral counting, recent movements have been toward some form of normalization that depends on extracting information from MS2 fragment ion intensities (e.g. SINQ<http://www.ncbi.nlm.nih.gov/pubmed/21656681> , SIn <http://www.ncbi.nlm.nih.gov/pubmed/20010810>,NSI/NSMT<http://www.ncbi.nlm.nih.gov/pubmed/22582177>). It appears that the CPFP at UT Southwestern has included SINQ quantitation as part of their dist via a perl module. I'm wondering whether there are any tools/scripts available on regis that would allow for the extraction of MS2 fragment ion intensity values or if this was in consideration/development at all?
Thanks for any thoughts and comments on this approach! Greg C. -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at http://groups.google.com/group/spctools-discuss?hl=en. For more options, visit https://groups.google.com/groups/opt_out.
