Kierstin,
If you're still looking to do this try the following X!Tandem parameters:
<note type="input" label="protein, cleavage site">[DE]|{P}</note>
<note type="input" label="protein, cleavage semi">yes</note>
DT
On Wednesday, July 24, 2013 1:35:26 PM UTC-5, Kierstin wrote:
>
> My experiment required digesting my proteins with Glu-C rather than
> trypsin. I'd like to modify my X!tandem parameters file to search for
> semi-Glu-C fragments. Does anyone have advice on how to do this? Thanks
> so much!
>
> Kierstin
>
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