Dear Luis,

I just uploaded a new release of MASSyPup, with TPP v4.7, Petunia GUI, X!Tandem workflow and Comet 14.01.rev0 working.
Any comments are welcome.

Best Regards,

Robert


On 14.05.2014 15:38, Luis Mendoza wrote:
That's great, Robert!  Thanks for including TPP in MASSyPup.

Does that mean that you are also including the Comet search engine, since we bundle it with TPP? Let us know if we can help you iron out those bugs with viewing those intermediate files.

Cheers,
--Luis


On Sun, Mar 16, 2014 at 6:45 AM, Robert <[email protected] <mailto:[email protected]>> wrote:

    Dear colleagues,

    the TPP is now portable. The 2014/03/15 release of MASSyPup
    (http://lababi.bioprocess.org/index.php/lababi-software/78-massypup)
    contains a working Petunia/TPP installation. I tested the
    X!Tandem->PepXML->PeptideProphet->ProteinProphet workflow +
    viewing. The test files are included.
    Both, USB and DVD versions of TPP-MASSyPup, work for me on a 2Gb
    and a 8Gb RAM computer.
    I had to apply plenty of tweaks to get the apache2 running and the
    custom compiled TPP funcional. Some comments: If a Perl script
    does not work, adding '/usr/bin/perl' in the first line usually
    solves the problem.
    There was no .password file in the release and there is no 'crypt'
    command in Puppy Linux/ Precise Debian. So I disabled the Password
    check in tpp_gui.pl <http://tpp_gui.pl> with the comment #RW. Some
    intermediate .xml files do not open, because the interface of the
    Viewing Utility has some wrong links (a '/tpp/' missing in the
    address), but the important stuff (tandem database search, pepXML,
    Peptide/ProteinProphet, viewing results) works fine.
    If you plan to remaster MASSyPup yourself, you have to copy the
    following files manually from /etc to /tmp/etc during the
    re-master process: passwd, group (I had to edit them manually for
    adding the www-data user) and the apache2 directory (which
    contains the Apache and TPP configuration).
    Please share your experiences (also pleasant ones) and bug fixes!

    Have fun, Robert
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