Yes indeed...the suffix prefix distinction is very important.  All top
decoy tools expect decoys to be labeled with prefix.

David
On May 20, 2014 11:19 PM, "钟传奇" <[email protected]> wrote:

> Hi, I have used Mayu integrated in TPP to analyze my data.
> The log file of Mayu showed that there is no decoy proteins found, and the
> log file is in the attachment.
> I generated my database for searching with other tools than the "
> reverse_fasta.pl" tool in the Mayu, and set the parameter "Tag (prefix)
> for decoy proteins" as "_REVERSED".
> I am wondering that the decoy proteins in my database fasta is
> tagged(Suffix) "_REVERSED", has this any effect on Mayu performance?
>
> Any suggestion will be welcomed.
>
> Thanks.
>
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