Thank David for reply.
So the only way is that I have to search my data against the database with
decoy proteins tagged prefix "rev_".


On Wed, May 21, 2014 at 11:23 PM, David Shteynberg <
[email protected]> wrote:

> Yes indeed...the suffix prefix distinction is very important.  All top
> decoy tools expect decoys to be labeled with prefix.
>
> David
> On May 20, 2014 11:19 PM, "钟传奇" <[email protected]> wrote:
>
>> Hi, I have used Mayu integrated in TPP to analyze my data.
>> The log file of Mayu showed that there is no decoy proteins found, and
>> the log file is in the attachment.
>> I generated my database for searching with other tools than the "
>> reverse_fasta.pl" tool in the Mayu, and set the parameter "Tag (prefix)
>> for decoy proteins" as "_REVERSED".
>> I am wondering that the decoy proteins in my database fasta is
>> tagged(Suffix) "_REVERSED", has this any effect on Mayu performance?
>>
>> Any suggestion will be welcomed.
>>
>> Thanks.
>>
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