Hi Eileen, I do not know the answer to your question. I do not see anything
in the documentation to suggest that this is the case. But do note that
xinteract sets a default lower bound the length which you need to change,
and there are some spectrast settings that may discard such spectra since
they are outside the normal bounds for most experiments. See the SpectraST
documentation here:



http://tools.proteomecenter.org/wiki/index.php?title=Software:SpectraST



Search for the “SPECTRUM FILTERING OPTIONS” section.



Regards,

Eric





*From:* [email protected] [mailto:
[email protected]] *On Behalf Of *Eileen Yue
*Sent:* Thursday, April 09, 2015 10:11 AM
*To:* [email protected]; [email protected]
*Cc:* [email protected]
*Subject:* [spctools-discuss] how to build/add small peptide (4-6amino
acid) library?







*Hello,*

May I ask one question related building library with Spectrast?



I am using  Windows command to build the library. There is one question:
whether Spectrast will eliminate all the peptide which is less than 6 amino
acid no matter the high  peptide phrophet? I am quite interested in some
small peptide which is only 4-5 amino acid and would like using SpectraSt
search to identify these small peptides. Is there any way that I could add
these small peptide CID spectra to library?



Thank you

Eileen

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