Hi I cannot create spectral library when I use the merged mzXML and pep.xml file generated from multiple raw files on PD1.4.
For example, there are two raw files, A.raw and B.raw. I analyzed this two raw files with Proteome Discoverer 1.4 (PD1.4) all together to generate one merged files, All.mzML file and All.msf file. This All.mzML file was converted to All.mzXML file by MSConvert. All.msf file was used to export All.pep.xml file on PD1.4. In All.pep.xml file, I replaced percolator and percolator_result with peptideprofet and peptideprofet_result, respectively. Finally, I tried to create spectral library from All.pep.xml file and All.mzXML file by using prompt command of SpectraST on Windows, but it didn't work. I successfully created a library when I processed two raw files separately. So, when I use merged files generated from multiple raw data on PD1.4, there are some problems. Is there any way to solve this problem? Thank you. waovoo -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at http://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
