Hi Jimmy, Thank you so much! The ProteoWizard group contributes a lot for the community.
Best, Cheng. On Fri, Jun 24, 2016 at 11:53 AM, Jimmy Eng <[email protected]> wrote: > Yes, it's normal because you are writing out a text file with lots of > numbers hence the larger size. That's why plain text is very inefficient > from a storage standpoint. For centroiding, do a google search for > "msconvert centroid". You can also search the ProteoWizard support archive: > > https://sourceforge.net/p/proteowizard/mailman/proteowizard-support/ > > Also here's some documentation on msconvert with a centroiding example: > > http://proteowizard.sourceforge.net/tools/msconvert.html > > > On Thu, Jun 23, 2016 at 7:49 PM, cheng chang <[email protected]> > wrote: > >> Hi Jimmy, >> >> Thanks for your reply. The new version of msconvert does work! However, >> when I convert my raw file (profile mode) to mgf file, I choose the >> "peakPicking" filter using "vendor msLevel=1-2" parameter to get the >> centroid mgf file. >> Well, for a 1GB file, I got 2.6GB mgf file at last. Is this normal? How >> to set parameters when users would like to get the centroid mzML or mgf >> file? >> >> Thanks in advance >> >> Cheng. >> >> On Fri, Jun 24, 2016 at 4:15 AM, Jimmy Eng <[email protected]> wrote: >> >>> You should try with the most recent version of msconvert ( >>> http://proteowizard.sourceforge.net/downloads.shtml) and if you still >>> get errors, post to the msconvert developers at the ProteoWizard help site: >>> [email protected] >>> >>> Attached a link to one of your raw files (Dropbox?) so that their >>> developers can debug with your data. >>> >>> On Thu, Jun 23, 2016 at 1:09 AM, cheng chang <[email protected]> >>> wrote: >>> >>>> Hi all, >>>> >>>> Recently, I used msconvert to convert the Fusion and Lumos raw file to >>>> mgf file in our lab. >>>> >>>> However, an error is found during the convertion. Here is the screen >>>> shot. Any help is appreciated! >>>> >>>> >>>> Best wishes, >>>> >>>> Cheng. >>>> >>>> >>>> <https://lh3.googleusercontent.com/-j5Oprt9kjLo/V2uXci3hIUI/AAAAAAAAAAM/NhFAdukJYbccaijVDJNTepTtlJQ9hTX1ACLcB/s1600/msconvert.png> >>>> >>>> -- >>>> You received this message because you are subscribed to the Google >>>> Groups "spctools-discuss" group. >>>> To unsubscribe from this group and stop receiving emails from it, send >>>> an email to [email protected]. >>>> To post to this group, send email to [email protected]. >>>> Visit this group at https://groups.google.com/group/spctools-discuss. >>>> For more options, visit https://groups.google.com/d/optout. >>>> >>> >>> >> >> >> -- >> Beijing Proteome Research Center >> State Key Laboratory of Proteomics >> Beijing,102206, China >> > > -- Beijing Proteome Research Center State Key Laboratory of Proteomics Beijing,102206, China -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
