Dear TPP members, How to calculate the fold change values in LIBRA quantification result? According to my understanding, the mean value in channel like 115 (treatment group) divided by mean value in channel 114 ( this my control sample) . If i am wrong, kindly tell me the correct procedure?
1 sp|D0PRN3|NRX3A_CHICK tr|F1NLI2|F1NLI2_CHICK *1.0000* max coverage: 3.5% LIBRA (4) 114.0: 0.33 ± 0.02 115.0: 0.26 ± 0.02 116.0: 0.23 ± 0.03 117.0: 0.16 ± 0.02 num unique peps: 3 tot indep spectra: 5 share of spectrum ids: 0.04% >Neurexin-3 OS=Gallus gallus PE=1 SV=1 Length: 1693aa >Neurexin-3-beta OS=Gallus gallus GN=NRXN3 PE=4 SV=2 *weight* *peptide sequence* *nsp adj prob* *init prob* *ntt* *nsp* *total* *pep grp ind* * wt-1.00 LELSRLARIGDT <http://localhost/tpp-bin/PepXMLViewer.cgi?page=1&requiredPeptideText=LELSRLARIGDT&xmlFileName=c:/Inetpub/wwwroot/ISB/data/parameters/interact.ipro.pep.xml> 0.9990 0.9990 1 2.06 1 --2_n145LELSRLARIGDT <http://localhost/tpp-bin/PepXMLViewer.cgi?page=1&requiredPeptideText=LELSRLARIGDT&xmlFileName=c:/Inetpub/wwwroot/ISB/data/parameters/interact.ipro.pep.xml&requiredSpectrumText=%5C.2%24> * wt-1.00 MGSISFDFRT <http://localhost/tpp-bin/PepXMLViewer.cgi?page=1&requiredPeptideText=MGSISFDFRT&xmlFileName=c:/Inetpub/wwwroot/ISB/data/parameters/interact.ipro.pep.xml> 0.9990 0.9990 1 2.06 1 --2_n145MGSISFDFRT <http://localhost/tpp-bin/PepXMLViewer.cgi?page=1&requiredPeptideText=MGSISFDFRT&xmlFileName=c:/Inetpub/wwwroot/ISB/data/parameters/interact.ipro.pep.xml&requiredSpectrumText=%5C.2%24> * wt-1.00 LSLVDGR <http://localhost/tpp-bin/PepXMLViewer.cgi?page=1&requiredPeptideText=LSLVDGR&xmlFileName=c:/Inetpub/wwwroot/ISB/data/parameters/interact.ipro.pep.xml> 0.9097 0.9076 1 2.15 3 --2_n145LSLVDGR <http://localhost/tpp-bin/PepXMLViewer.cgi?page=1&requiredPeptideText=LSLVDGR&xmlFileName=c:/Inetpub/wwwroot/ISB/data/parameters/interact.ipro.pep.xml&requiredSpectrumText=%5C.2%24> * wt-1.00 TPFTASGESE <http://localhost/tpp-bin/PepXMLViewer.cgi?page=1&requiredPeptideText=TPFTASGESE&xmlFileName=c:/Inetpub/wwwroot/ISB/data/parameters/interact.ipro.pep.xml> 0.1498 0.1498 1 2.91 1 --2_n145TPFTAS167GES167E <http://localhost/tpp-bin/PepXMLViewer.cgi?page=1&requiredPeptideText=TPFTASGESE&xmlFileName=c:/Inetpub/wwwroot/ISB/data/parameters/interact.ipro.pep.xml&requiredSpectrumText=%5C.2%24> -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
