Hi everybody,
We are running dimethylation experiments with platelet proteom. Therefore, we using light and intermediate formaldehyde for labeling and we are quit happy about the labeling efficiency and the number of unique peptides. Unfortunately we get some XPress Ratios with "-1". For some of those values it is possible to gain the correct ratio when refreshing peptide prophet, but not for every peptide. I will give a screenshot of the parameter settings: <https://lh3.googleusercontent.com/--7lsaZ6i2P8/V4vVH2CJFBI/AAAAAAAAAAM/pFxPzQ59tU0f_hXNRXufMQfLrvP9zzLiwCLcB/s1600/heavy%2Bdimethylated.jpg> Furthermore, we are using mascot for search and set as fixed modifications: Light or intermediate dimethylation. We highly appreciated your support or any idea. Greetings, Max -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
