Hello everyone, 

I was wondering how does CLIPPER work (the add-on of TPP) to plot and draw 
the gaussian in order to determine the 3 sigma value ? 
What are the peptides used to that end ? Only the naturally blocked or all 
the iTRAQ-labelled peptides ? 

Can anyone do the same analysis manually (it seems CLIPPER doesn't work 
with the TPP V5.0 but maybe it comes from my computer as I am not a pro ! )?

Thanks for any answer

Cedric Leduc

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