Dear all

I would like to compare the identification rate of a set of 
PeptideProphet/iProphet validated results from Comet/X!Tandem, with 
MaxQuant's results. For MaxQuant I have PEPs. What is the fair way to 
compare the results with these two types of validations? At what threshold 
should I cut the peptideprohet/iprophet to be comparable with a PEP of say 
0.1?

Is it fair if I compare a PEP=x with PepPro/iPro=1-x? One thing that I have 
noticed is that FDR calculated by mean(all PEPs) OR 1-mean(all PepPro), is 
always much less for PepPro.

And one more question, what is an acceptable PepPro/iPro probability? Is 
there a way that I allow a higher FDR without changing the probability 
threshold?

Thank you very much in advance.

Ali

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