Dear All, my general question would be whether it is possible to build a spectral library with spectrast starting from ProteinPilot search file.
Specifically, I have converted ProteinPilot .group file to mzid with PP itself, then to pepXML with "idconverter" Underlying .wiff file was converted with ABSciex_MS_Converter to .mzML and located in the same directory as .pepXML file. Now, when I supply my .pepXML file to "PeptideProphet" an error appears: > > C:\WINDOWS\system32>c:\TPP\bin\xinteract.exe g:\TPP\data\EEXL1-2_SG_AP_1_annot4_mod2.pep.xml c:\TPP\bin\xinteract.exe (TPP v5.0.0 Typhoon, Build 201610111222-7423 (Windows_NT-x86_64)) running: "C:/TPP/bin/InteractParser "C:/tmp/a13260/interact.pep.xml" "g:\TPP\data\EEXL1-2_SG_AP_1_annot4_mod2.pep.xml" -L"7"" terminate called after throwing an instance of 'boost::exception_detail::clone_impl<boost::exception_detail::error_info_injector<std::ios_base::failure[abi:cxx11]> >' what(): failed opening file: ═х єфрхЄё эрщЄш єърчрээ√щ Їрщы. : iostream error This application has requested the Runtime to terminate it in an unusual way. Please contact the application's support team for more information. command "C:/TPP/bin/InteractParser "C:/tmp/a13260/interact.pep.xml" "g:\TPP\data\EEXL1-2_SG_AP_1_annot4_mod2.pep.xml" -L"7"" failed: No such process command "C:/TPP/bin/InteractParser "C:/tmp/a13260/interact.pep.xml" "g:\TPP\data\EEXL1-2_SG_AP_1_annot4_mod2.pep.xml" -L"7"" exited with non-zero exit code: 3 QUIT - the job is incomplete > > More specified input (c:\TPP\bin\xinteract.exe g:\TPP\data\EEXL1-2_SG_AP_1_annot4_mod2.pep.xml -Dg:\TPP\data\20161219_XL-XT_Uniprot_iRT_SUMO_NB_cRAP_annot4.fasta -ag:\TPP\data\ -NEEXL1-2_SG_AP_1_Interact.pep.xml -p0.99 -dRRRRR -ORAPd ) produces the same error. I would really appreciate any help in fixing it if it is possible. Thanks a lot in advance, Ivan -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
