Hi Adam, Thank you for your report. We have now modified how StPeter handles modifications. If you build TPP from sources, you can see this in rev 7675. Or let me know if you require a Windows executable, and we'll post it on our files section.
Cheers, --Luis On Thu, Nov 9, 2017 at 8:51 AM, Adam R <[email protected]> wrote: > Hi Everyone, > > I've tried to run StPeter on a prox.xml file from a sample that was > labeled with TMT 126. This was not quantified with Libra, just trying to > identify peptides, so I set the 126 mass as a fixed mass for lysine under > fixed modifications and searched with comet. I have peptide IDs and may > proteins identified with this approach, showing K[254] for the amino acid > and the mod. I figured that setting up StPeter this way shouldn't an issue > since this can be done with other fixed mods like with cysteine. Any > thoughts? > > Best, > > Adam > > > StPeter: MS2 intensity-based label-free quantification > Copyright 2015-2017, Jason Winget, Michael Hoopmann, Institute for Systems > Biology > Version 1.2.0 October 11 2017 > ****** BEGIN StPeter ANALYSIS ****** > Time at start of analysis: Thu Nov 09 16:46:36 2017 > > Parameters: > degenerate peptides = no > fdr = 0.01 > sample load = 0 > tolerance = 0.4 > intensities = no > > Reading protXML: c:/TPP/data/20171108_EL-2017-00005268/126/interact.ipro. > prot.xml > ERROR: Unknown modification: K[254] > Please report to authors. > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to [email protected]. > To post to this group, send email to [email protected]. > Visit this group at https://groups.google.com/group/spctools-discuss. > For more options, visit https://groups.google.com/d/optout. > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
