Hi Luis, Thanks for getting back on this. I realized also that I was searching using the wrong mod and should have used 229.16 and not the reporter ion mass! I will need a windows executable file please.
Best, Adam On Friday, November 10, 2017 at 2:06:30 PM UTC-6, Luis wrote: > > Hi Adam, > > Thank you for your report. We have now modified how StPeter handles > modifications. If you build TPP from sources, you can see this in rev > 7675. Or let me know if you require a Windows executable, and we'll post > it on our files section. > > Cheers, > --Luis > > > On Thu, Nov 9, 2017 at 8:51 AM, Adam R <[email protected] <javascript:>> > wrote: > >> Hi Everyone, >> >> I've tried to run StPeter on a prox.xml file from a sample that was >> labeled with TMT 126. This was not quantified with Libra, just trying to >> identify peptides, so I set the 126 mass as a fixed mass for lysine under >> fixed modifications and searched with comet. I have peptide IDs and may >> proteins identified with this approach, showing K[254] for the amino acid >> and the mod. I figured that setting up StPeter this way shouldn't an issue >> since this can be done with other fixed mods like with cysteine. Any >> thoughts? >> >> Best, >> >> Adam >> >> >> StPeter: MS2 intensity-based label-free quantification >> Copyright 2015-2017, Jason Winget, Michael Hoopmann, Institute for >> Systems Biology >> Version 1.2.0 October 11 2017 >> ****** BEGIN StPeter ANALYSIS ****** >> Time at start of analysis: Thu Nov 09 16:46:36 2017 >> >> Parameters: >> degenerate peptides = no >> fdr = 0.01 >> sample load = 0 >> tolerance = 0.4 >> intensities = no >> >> Reading protXML: >> c:/TPP/data/20171108_EL-2017-00005268/126/interact.ipro.prot.xml >> ERROR: Unknown modification: K[254] >> Please report to authors. >> >> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to [email protected] <javascript:>. >> To post to this group, send email to [email protected] >> <javascript:>. >> Visit this group at https://groups.google.com/group/spctools-discuss. >> For more options, visit https://groups.google.com/d/optout. >> > > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
