Hello everyone, I'm new guy in M.S, recently I try to learn how to process MS data using TPP pipe line and follow the TPP tutorial. I installed the TPP v5.1.0. As I went on to step 9 which is peptide quantitation with ASAPRatio. This is what they said to set up the parameters. *ASAPRatio is a tool for measuring relative expression levels of peptides and proteins from isotopically-labeled samples (e.g. ICAT, SILAC, etc).* - *Click on the Analyze Peptides tab under the Analysis Pipeline section in Petunia to access the xinteract interface again.* - *Select the interact.ipro.pep.xml file in the directory demo. Make sure that there is only one file selected for analysis; you can edit the selections using the checkboxes and Remove button on the right-hand side.*
*Important**Under Output and Filter Options, change "Write output to file:" to have the same name as the input file, interact.ipro.pep.xml**Under PeptideProphet Options**Make sure that you uncheck the option to RUN PeptideProphet**Under "Enter additional options to pass directly to the command-line (expert use only!)", enter the text: -nI**Under ASAPRatio Options**select to RUN ASAPRatio, change Labeled Residues to K and R**set m/z range to include in summation of peak to 0.05**set Specified masses to M 147.035, K 136.10916, and R 166.10941* - *Leave all other options set to their defaults, and click on Run XInteract at the bottom of the page to run ASAPRatio* However, after running program, I got this information: *EXECUTING: cd c:/TPP/data/demo && c: && C:/TPP/bin/xinteract -Ninteract.ipro.pep.xml -p0.05 -l7 -nI -nP -A-lKR-r0.05-mM147.035K136.10916R166.10941 interact.ipro.pep.xml * C:/TPP/bin/xinteract (TPP v5.1.0 Syzygy, Build 201711031215-7670 (Windows_NT-x86_64)) INFO: -nI option enabled InteractParser will not be utilized. running: "C:/TPP/bin/DatabaseParser "/tmp/a20904/demo/interact.ipro.pep.xml"" This application has requested the Runtime to terminate it in an unusual way. Please contact the application's support team for more information.error, could not execute: C:/TPP/bin/DatabaseParser "/tmp/a20904/demo/interact.ipro.pep.xml" Does anybody know what should I do to run this? Thank you! -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
