Thanks guys.
Could you please briefly comment on how to interpret the StPeter Sin value? The 
lower, the more abundant or the other way around?

Have a nice day

Filippo 

Da: Michael Hoopmann
Inviato: giovedì 14 dicembre 2017 21:15
A: [email protected]
Oggetto: RE: [spctools-discuss] Re: StPeter documentation

Manuscript is in review, but documentation is already under construction: 
http://tools.proteomecenter.org/wiki/index.php?title=Software:StPeter
Cheers,
Mike

From: [email protected] 
[mailto:[email protected]] On Behalf Of Filippo GENOVESE
Sent: Monday, December 11, 2017 4:51 AM
To: spctools-discuss
Subject: Re: [spctools-discuss] Re: StPeter documentation

Any news?

Filippo

Il giorno venerdì 14 luglio 2017 01:39:01 UTC+2, Hampton, Brian ha scritto:
Thanks Jason!  Once your manuscript is published it would be great to announce 
that on this list. 

Cheers!

Brian

On Jul 12, 2017, at 8:13 AM, Jason Winget <[email protected]> wrote:
Hi Brian, 
StPeter is a direct implementation of the Normalized Spectral Index, described 
by Griffin et. al: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2805705/
It uses MS2 intensities for protein-level quantification.

We are working on publishing it independently but so far journal editors have 
not been very receptive...

As for usage, if you run StPeter from the command line with no arguments you 
will see a usage statement. By default it uses only non-degenerate peptides and 
a 1% FDR cutoff. Typically the default values are fine, so you should just be 
able to run it against your ProtXML results. It will write the quantification 
values back into the ProtXML and will also output some simple CSV results.

At the moment PTMs are hard-coded and contain only a limited set, so the most 
common issue that I run into is that the program will crash when searching 
"unusual" mods.

-Jason

On Friday, June 16, 2017 at 5:33:59 PM UTC-4, Brian Hampton wrote: 
Hello, 

I would be appreciative if someone could point me to documentation that 
describes StPeter which is used for label-free quantification in TPP. I've 
tried the usual searching with Google, PubMed and looking through the SPC Tools 
sites without success. 

Thanks, 

Brian 
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