Hi all,


I’m a bit stuck on importing an existing PeakView library (tsv or txt 
format) into SpectraST.

In the log file from SpectraST it says the following :


START: (Mon Jul 16 14:16:51 2018) spectrast -cNimported out.tsv 

GENERAL: File offset size is 8 bytes. Big library supported.

GENERAL: Pointer size is 8 bytes. 

TSV IMPORT: Peptide ID too short. Skipped spectrum 778.427.427 .

TSV IMPORT: Peptide ID too short. Skipped spectrum 778.498.498 .

TSV IMPORT: Peptide ID too short. Skipped spectrum 778.569.569 .

TSV IMPORT: Peptide ID too short. Skipped spectrum 778.214.214 .

…

CREATE: IMPORT FROM TSV 
"c:/TPP/data/dbase/speclibs/test/out.tsv"[P=0.9;n=;g=FALSE;o=FALSE;I=;_RNT=0;_RDR=100000;_DCN=0;_NAA=6;_NPK=10;_CEN=FALSE;_XAN=FALSE]

TSV IMPORT: Total of 0 spectra imported, 3531720 spectra skipped.

PERFORMANCE: Total Run Time = 54 seconds.

END: (Mon Jul 16 14:17:45 2018) spectrast -cNimported out.tsv


The column formats I've tried are:

Q1    Q3    RT_detected    protein_name    isotype    relative_intensity    
stripped_sequence    modification_sequence    prec_z    frg_type    
frg_z    frg_nr    iRT    uniprot_id    decoy    N    confidence    shared


and 


PrecursorMz    ProductMz    Tr_recalibrated    transition_name    CE    
LibraryIntensity    transition_group_id    ProteinName    GroupLabel    
PeptideSequence    FullUniModPeptideName    UniprotID    decoy    shared    
confidence    PrecursorCharge    FragmentType    FragmentCharge    
FragmentSeriesNumber

 

Any ideas?



Moritz

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