Hi Kamal,

Yes, you can use PeptideProphet to validate Kojak results. Select “Kojak” from 
your pipeline in the upper right of the TPP page, and it will default 
PeptideProphet parameters for Kojak. Input is the PepXML output from Kojak.

 

The windows version of Percolator can be found here: 
https://github.com/percolator/percolator/releases

To use it in the TPP do the following:

1.      Install Percolator on your computer.

2.      Go into the Percolator bin folder (usually something like C:\Program 
Files\Percolator\bin\) and copy percolator.exe

3.      Paste it into your TPP bin folder (usually something like C:\TPP\bin\)

4.      In the TPP, select “External Tools->Percolator” from the menu. Input is 
the .perc.txt output files from Kojak.

 

Cheers,

Mike

 

From: spctools-discuss@googlegroups.com 
[mailto:spctools-discuss@googlegroups.com] On Behalf Of Kamal Mandal
Sent: Thursday, September 27, 2018 12:17 PM
To: spctools-discuss
Subject: [spctools-discuss] Kojak

 

Hello,

Is it possible to use PeptideProphet for validation of data (Cross-linked 
peptides) obtained from Kojak search in TPP.

Is the windows version of percolator available and how do we integrate that to 
TPP suite?

 

Thanks,

 

-Kamal

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