Hi Alex,

What is the content of your PATH environment variable?  You can check with
echo $PATH command.  You should add the directory with TPP binaries (
/usr/local/tpp/bin ) to you PATH.  The way to do this depends on your
shell.

In bash shell do this:
export PATH=/usr/local/tpp/bin:$PATH

In C shell do this:
setenv PATH /usr/local/tpp/bin:$PATH

Let me know if it still gives the same message after changing the PATH.

-David


On Tue, Oct 2, 2018 at 9:24 AM Alex Zelter <[email protected]> wrote:

> Hi there,
>
> I am using TPP v5.2.0-b1 Flammagenitus Pre-release Beta, Build
> 201809101205-7809 (Linux-x86_64) with Kojak pep.xml data running the
> following command:
>
> /usr/local/tpp/bin/xinteract -Ninteract.pep.xml -p0 -l1 -eT -PPM -OAPdp
> -drandom_ -ip file1.pep.xml file3.pep.xml file3.pep.xml
>
> This command runs to completion, however looking in the output I see:
>
> ....
> running: "/usr/local/tpp/bin/ProphetModels.pl -i interact.pep.xml -d
> "random_""
> Analyzing interact.pep.xml ...
> ....
>   => Total so far: 134784 hits. (3317 decoys, 0 excluded)
> Using decoy ratio of 0.0284042151512031 for PeptideProphet.
> Inserting decoy analysis tags in line 4542 of interact.pep.xml
> Running tpp_models...
> *Can't exec "tpp_models.pl <http://tpp_models.pl>": No such file or
> directory at /usr/local/tpp/bin/ProphetModels.pl line 1179.*
> Done
> command completed in 35 sec
> ...
>
> Should I be concerned by this? I get this error twice during the run.
> Later in the output the same command is run but this time a filename is
> given to tpp_models.pl and the program then runs with no errors:
>
>
> running: "/usr/local/tpp/bin/tpp_models.pl '/path/to/interact.pep.xml'"
> File: /path/to/interact.pep.xml
>  - in ms run: /path/to/file1...
>  - in ms run: /path/to/file2...
>  - in ms run: /path/to/file3...
>
> -------------------------------------------------------------------------------
> TPP DASHBOARD -- started at Mon Oct  1 16:03:50 2018
>
> -------------------------------------------------------------------------------
> File /path/to/interact.pep.xml is pepxml
> Found fval (+1) model...
> etc...
>
> tpp_models.pl is present and executable on the computer's filesystem so I
> assume this error is due to tpp_models.pl not being given a file to
> operate on in the instances where there is a "no such file" error.
>
> Am I safe to ignore this?
> Thanks!
> Alex
>
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