Hi Goutham,

The problem you have is likely fixed in the next release of the TPP (due in a 
few weeks). Or you can grab it from the release candidates we currently offer.

Cheers,

Mike

 

From: [email protected] 
[mailto:[email protected]] On Behalf Of Goutham Vasam
Sent: Thursday, February 21, 2019 11:31 AM
To: spctools-discuss
Subject: [spctools-discuss] StPeter ERROR: Unknown modification: C[158]

 

Hi,

While running StPeter on TPP, I received an error with following details:

StPeter: MS2 intensity-based label-free quantification
Copyright 2015-2017, Jason Winget, Michael Hoopmann, Institute for Systems 
Biology
Version 1.2.0 October 11 2017
****** BEGIN StPeter ANALYSIS ******
 Time at start of analysis: Thu Feb 21 14:23:02 2019
 
 Parameters:
  degenerate peptides = no
  fdr = 0.01
  sample load = 0
  tolerance = 0.4
  intensities = no
 
 Reading protXML: 
c:/TPP/data/Goutham/Proteomics_Run_December_2018_B_AA_30_60_120min_Raw_data/All_modifications/AA120_35_1/interact.ipro.prot.xml
ERROR: Unknown modification: C[158]
Please report to authors.
 

Command FAILED
RETURN CODE:256

I have attached the XML file and comet.params file. I emailed Dr. Hoopmann (as 
it said report to authors). If there are alternate ways, I would appreciate any 
help. Thank you!

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