Hey David! Yes, I'd like to know more about DISCo. I was poking around that tool too and kinda got lost :) That's what 3 years away from proteomics will do for you! The MSconvert within TPP creates mzML files fine, I guess I don't know how to check to see if they have been demultiplexed. Can I use them in DISCo or should I use the MS2 fragment extractor?
-JN On Thu, 22 Oct 2020 at 16:17, 'David Shteynberg' via spctools-discuss < [email protected]> wrote: > Dear Jane, > > I cannot help you with the demultiplexing, because I have not used that > feature before in MSConvert. But if you do manage to get a regular DIA > mzML file out of the conversion you can use the DISCo tool available in the > TPP under Tools->DIA->Extract MS2 Fragments that will generate a new mzML > file that you can search and analyze with DDA tools. As DISCo has recently > seen major improvements I can provide you with a release candidate version > if you are interested. > > Cheers, > -David > > On Thu, Oct 22, 2020 at 1:08 PM jane n <[email protected]> wrote: > >> Hi all, >> >> I have acquired a bunch of files on my Eclipse to build a DIA library >> using an 8mz window in overlap mode (ie essentially 4mz windows) and I'm >> not clear if the Convert to MzML is demultiplexing to 4mz windows? I've >> tried doing it manually in MSconvert but the conversion fails. On the >> other hand, do I need to demulitplex? >> >> Cheers! >> -Jane >> >> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to [email protected]. >> To view this discussion on the web visit >> https://groups.google.com/d/msgid/spctools-discuss/af9c2037-59d0-478e-bcfc-d0d09adabd29n%40googlegroups.com >> <https://groups.google.com/d/msgid/spctools-discuss/af9c2037-59d0-478e-bcfc-d0d09adabd29n%40googlegroups.com?utm_medium=email&utm_source=footer> >> . >> > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to [email protected]. > To view this discussion on the web visit > https://groups.google.com/d/msgid/spctools-discuss/CAGJJY%3D97cbNtG3XjUbopu-seY-rU4bj8peX-UG1cL948Tgi17g%40mail.gmail.com > <https://groups.google.com/d/msgid/spctools-discuss/CAGJJY%3D97cbNtG3XjUbopu-seY-rU4bj8peX-UG1cL948Tgi17g%40mail.gmail.com?utm_medium=email&utm_source=footer> > . > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/CAL-xMntWrAeuTJqOL69rOZf3wRJO2hOmvUuthThmSo6d4ag7cw%40mail.gmail.com.
