Jane, See this from the Skyline team: https://skyline.ms/wiki/home/software/Skyline/page.view?name=DemultiplexingOverlappingDIAWindows
Best Mark On Thu, Oct 22, 2020 at 4:28 PM Science Gurl <[email protected]> wrote: > Hey David! > > Yes, I'd like to know more about DISCo. I was poking around that tool too > and kinda got lost :) That's what 3 years away from proteomics will do for > you! The MSconvert within TPP creates mzML files fine, I guess I don't > know how to check to see if they have been demultiplexed. Can I use them > in DISCo or should I use the MS2 fragment extractor? > > -JN > > On Thu, 22 Oct 2020 at 16:17, 'David Shteynberg' via spctools-discuss < > [email protected]> wrote: > >> Dear Jane, >> >> I cannot help you with the demultiplexing, because I have not used that >> feature before in MSConvert. But if you do manage to get a regular DIA >> mzML file out of the conversion you can use the DISCo tool available in the >> TPP under Tools->DIA->Extract MS2 Fragments that will generate a new mzML >> file that you can search and analyze with DDA tools. As DISCo has recently >> seen major improvements I can provide you with a release candidate version >> if you are interested. >> >> Cheers, >> -David >> >> On Thu, Oct 22, 2020 at 1:08 PM jane n <[email protected]> wrote: >> >>> Hi all, >>> >>> I have acquired a bunch of files on my Eclipse to build a DIA library >>> using an 8mz window in overlap mode (ie essentially 4mz windows) and I'm >>> not clear if the Convert to MzML is demultiplexing to 4mz windows? I've >>> tried doing it manually in MSconvert but the conversion fails. On the >>> other hand, do I need to demulitplex? >>> >>> Cheers! >>> -Jane >>> >>> -- >>> You received this message because you are subscribed to the Google >>> Groups "spctools-discuss" group. >>> To unsubscribe from this group and stop receiving emails from it, send >>> an email to [email protected]. >>> To view this discussion on the web visit >>> https://groups.google.com/d/msgid/spctools-discuss/af9c2037-59d0-478e-bcfc-d0d09adabd29n%40googlegroups.com >>> <https://groups.google.com/d/msgid/spctools-discuss/af9c2037-59d0-478e-bcfc-d0d09adabd29n%40googlegroups.com?utm_medium=email&utm_source=footer> >>> . >>> >> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to [email protected]. >> To view this discussion on the web visit >> https://groups.google.com/d/msgid/spctools-discuss/CAGJJY%3D97cbNtG3XjUbopu-seY-rU4bj8peX-UG1cL948Tgi17g%40mail.gmail.com >> <https://groups.google.com/d/msgid/spctools-discuss/CAGJJY%3D97cbNtG3XjUbopu-seY-rU4bj8peX-UG1cL948Tgi17g%40mail.gmail.com?utm_medium=email&utm_source=footer> >> . >> > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to [email protected]. > To view this discussion on the web visit > https://groups.google.com/d/msgid/spctools-discuss/CAL-xMntWrAeuTJqOL69rOZf3wRJO2hOmvUuthThmSo6d4ag7cw%40mail.gmail.com > <https://groups.google.com/d/msgid/spctools-discuss/CAL-xMntWrAeuTJqOL69rOZf3wRJO2hOmvUuthThmSo6d4ag7cw%40mail.gmail.com?utm_medium=email&utm_source=footer> > . > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/CABW2DNrj4nbxyaTYa9NBxoFfM0wYq1xOOczLvJ5T70RgYaEZfw%40mail.gmail.com.
