Hi Mike,

Thanks for getting back to me so quickly. Here's the 
database: https://we.tl/t-wZsZ3uSq1r

Is version 6 available as a container? I've never managed to install TPP 
properly on a linux machine ...

Alastair

On Monday, 21 June 2021 at 17:50:24 UTC+2 [email protected] wrote:

> Hi Alastair,
>
> There is a much newer version of tpp2mzid in our current release 
> candidates of TPP version 6. I’m hoping that the error messages have been 
> resolved in this new version. However, I also need the fasta search 
> database file to run the conversion with the newer tpp2mzid. Could you also 
> provide a link to that file and I will finish my tests and let you know the 
> result?
>
> Thanks,
>
> Mike
>
>  
>
> *From:* 'Alastair Skeffington' via spctools-discuss <
> [email protected]> 
> *Sent:* Monday, June 21, 2021 6:45 AM
> *To:* spctools-discuss <[email protected]>
> *Subject:* [spctools-discuss] tpp2mzid help - 'no peptide evidence found 
> ...'
>
>  
>
> I'm at the end of a multi-year odyssey of a project, and I just need to 
> submit data to PRIDE.
>
>  
>
> Unfortunately, when I run tpp2mzid for some prot.xml files, I get an error 
> such as this:
>
>  
>
> Warning (A), no peptide evidence found for (mod): EENAFVQKIAE from 
> Br20418.t2
>
>  
>
> This only happens for some prot.xml files, but I'm not sure why. The 
> pep.xml files from which the prot.xml are derived convert fine.
>
>  
>
> I've put an example here: https://we.tl/t-Z9qNNjDC0k
>
>  
>
> I was running TPP via the docker 
> container: digitalproteomes/tpp:version-5.2.0.1
>
>  
>
> Here is the stdout in full:
>
> Reading: HCLC_EhN.prot.xml
>
> Reading: /data/HCLC_EhN_ipro.pep.xml
>
> Unknown xx modification: B = 114.535 42.5515
>
> Unknown xx modification: J = 113.084 44.0023
>
> Unknown xx modification: O = 237.148 80.0613
>
> Unknown xx modification: U = 150.954 6.13277
>
> Unknown xx modification: X = 111 46.0864
>
> Unknown xx modification: Z = 128.551 28.5358
>
> Unknown xx modification: B = 114.535 42.5515
>
> Unknown xx modification: J = 113.084 44.0023
>
> Unknown xx modification: O = 237.148 80.0613
>
> Unknown xx modification: U = 150.954 6.13277
>
> Unknown xx modification: X = 111 46.0864
>
> Unknown xx modification: Z = 128.551 28.5358
>
> Unknown xx modification: B = 114.535 42.5515
>
> Unknown xx modification: J = 113.084 44.0023
>
> Unknown xx modification: O = 237.148 80.0613
>
> Unknown xx modification: U = 150.954 6.13277
>
> Unknown xx modification: X = 111 46.0864
>
> Unknown xx modification: Z = 128.551 28.5358
>
> Unknown xx modification: B = 114.535 42.5515
>
> Unknown xx modification: J = 113.084 44.0023
>
> Unknown xx modification: O = 237.148 80.0613
>
> Unknown xx modification: U = 150.954 6.13277
>
> Unknown xx modification: X = 111 46.0864
>
> Unknown xx modification: Z = 128.551 28.5358
>
> Unknown xx modification: B = 114.535 42.5515
>
> Unknown xx modification: J = 113.084 44.0023
>
> Unknown xx modification: O = 237.148 80.0613
>
> Unknown xx modification: U = 150.954 6.13277
>
> Unknown xx modification: X = 111 46.0864
>
> Unknown xx modification: Z = 128.551 28.5358
>
> Unknown xx modification: B = 114.535 42.5515
>
> Unknown xx modification: J = 113.084 44.0023
>
> Unknown xx modification: O = 237.148 80.0613
>
> Unknown xx modification: U = 150.954 6.13277
>
> Unknown xx modification: X = 111 46.0864
>
> Unknown xx modification: Z = 128.551 28.5358
>
> Unknown xx modification: B = 114.535 42.5515
>
> Unknown xx modification: J = 113.084 44.0023
>
> Unknown xx modification: O = 237.148 80.0613
>
> Unknown xx modification: U = 150.954 6.13277
>
> Unknown xx modification: X = 111 46.0864
>
> Unknown xx modification: Z = 128.551 28.5358
>
> Unknown xx modification: B = 114.535 42.5515
>
> Unknown xx modification: J = 113.084 44.0023
>
> Unknown xx modification: O = 237.148 80.0613
>
> Unknown xx modification: U = 150.954 6.13277
>
> Unknown xx modification: X = 111 46.0864
>
> Unknown xx modification: Z = 128.551 28.5358
>
> 17648 psms read.
>
> Warning (A), no peptide evidence found for (mod): EENAFVQKIAE from 
> Br20418.t2
>
>  
>
> Help would be very much appreciated!
>
> Thanks,
>
> Alastair
>
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