Dear all I am quantifying an iTRAQ run, where one of the channel seems to be having lower intensity across the board (around 20%). I am thinking of the TIC normalization (total intensity), but it is mentioned as "not recommended" in LIBRA settings.
Why is it so? If I am mixing equal labelled peptide, what is wrong in TIC, assuming one of the peptides labeled poorly? What is the alternative here if that is not suitable? Why is it so? If I am mixing equal labelled peptide, what is wrong in TIC, assuming one of the peptides labeled poorly? What is the alternative here if that is not suitable? *Also,* *Can I see the total intensity of each channel somewhere?* Regards Debojyoti -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/42859427-dc18-4b68-965c-84bf0cd68168n%40googlegroups.com.
