Can anyone help me with this, please? On Monday, July 31, 2023 at 11:39:47 PM UTC+5:30 Debojyoti Pal wrote:
> Dear all > > I am quantifying an iTRAQ run, where one of the channel seems to be having > lower intensity across the board (around 20%). I am thinking of the TIC > normalization (total intensity), but it is mentioned as "not recommended" > in LIBRA settings. > > Why is it so? If I am mixing equal labelled peptide, what is wrong in TIC, > assuming one of the peptides labeled poorly? What is the alternative here > if that is not suitable? > > Why is it so? If I am mixing equal labelled peptide, what is wrong in TIC, > assuming one of the peptides labeled poorly? What is the alternative here > if that is not suitable? > > *Also,* *Can I see the total intensity of each channel somewhere?* > > Regards > Debojyoti > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/deefbc54-2ac6-4b6b-9b53-df67c02336ban%40googlegroups.com.
