Scientists Monitor Bt Protein in Corn Ethanol

___________________________________________

ARS News Service
Agricultural Research Service, USDA
Jan Suszkiw, (301) 504-1630, [EMAIL PROTECTED]
July 5, 2002
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Protein from the soil bacterium Bacillus thuringiensis has earned much
praise for its environmental and economic benefits as a natural pesticide.
But less well known is what happens to the protein in Bt-modified corn
when processed into ethanol.

To find out, Agricultural Research Service (ARS) chemical engineer Bruce
Dien and colleagues designed small-scale experiments with wet- and
dry-milled Bt corn hybrids that enabled them to monitor the protein during
all stages of ethanol production.

According to Dien, with ARS' National Center for Agricultural Utilization
Research in Peoria, Ill., there's been little prior research delving into
Bt's effects on ethanol even though Bt-modified corn accounts for roughly
25 percent of the U.S. crop.

Bt corn contains genes from the bacterium for making the protein as a
built-in pesticide against the larvae of European corn borers. At each
stage of their experiments, Dien's team checked for the presence and
amount of the Bt protein, CRY1Ab, using an antibody-based test called an
Enzyme-Linked Immunosorbent Assay.

During dry milling, they found, the use of heat to liquefy corn meal
quickly destroyed the protein, and there was no detectable trace of it in
either the mash or resulting ethanol. In wet-milled corn, they detected
Bt in whole kernels, gluten, germ oil and fiber at concentrations of 170
to 453 parts per billion. But nothing turned up in the starch or steep
liquor fraction, used to produce the ethanol.

Ethanol yields from Bt corn also matched that of non-Bt hybrids. Typical
industrial ethanol yields are about 2.7 gallons per bushel via dry-milling
and 2.5 gallons for wet-milled corn.

More details about these studies, including new findings on the role of
corn starch on ethanol yields, appear in this month's issue of
Agricultural Research magazine, available on the web at:
http://www.ars.usda.gov/is/AR/archive/jul02/track0702.htm

ARS is the U.S. Department of Agriculture's chief scientific research
agency.

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