On Sat, Jan 26, 2013 at 1:46 PM, J. Forster <[email protected]> wrote: > The microscope group can help with reccomendations. 1000x is really > pushing it, because of 'empty magnification'. >
1000x is the "standard". Almost every microscope in a biology lab will have a 100x oil immersion objective and a 10x eyepiece. And then also have two or three lower power objectives as well. If you were to try and get 1000x from a 40x objective or if the optical quality were poor, yes, then you are just getting big, blurry images with no more detai than you'd see at 400x. That is what you call "empty magnification" and it is. It turns out that 1000x is about the limit of light microscopes and you pretty much need that if the goal is to see structures inside a cell, rather then just the cell's outline. You need to use oil that has about the same refractive index as the glass cover slide. In high school level labs they use cheaper 400x scopes and just look at larger stuff. Chris Albertson Redondo Beach, California _______________________________________________ time-nuts mailing list -- [email protected] To unsubscribe, go to https://www.febo.com/cgi-bin/mailman/listinfo/time-nuts and follow the instructions there.
