Hi Alireza,

Have you excluded interactions between bonded beads? One of your CG
molecule has 3 CG beads, so try excluding non-bonded interactions among
them and see if that works for you. You can use gromacs [exclusions]
setting in your topology to exclude the interactions.

Best,
Sikandar

On Tue, Feb 9, 2016 at 2:20 PM, Alireza Moradzadeh <[email protected]>
wrote:

> In fact, I did the same thing but it worked only for first step then it
> started to give me LINCS errors. Due to non equilibrated sys.
>
>
> > On Feb 9, 2016, at 12:24 PM, Christoph Junghans <[email protected]>
> wrote:
> >
> > 2016-02-08 17:40 GMT-07:00 Alireza Moradzadeh <[email protected]>:
> >> Dear Christoph,
> >>
> >> Thank you for your email. But I encountered problem with implementation
> of it. I want to use PME as my coulomb type. So I have to put my three bead
> molecule beads in different charge groups. But I face severe problem with
> electrostatic interaction between beads which blows up my system. I am
> seeking a way to equilibrate this system.
> >
> > If you need to combine PME with tabulated interactions, you might need
> > to start from a different initial guess for the potentials, because
> > the Boltzmann inversion of the g(r) contains contributions from the
> > coulombic part, too.
> >
> > I would use a couple of pure LJ interactions for a start (put them as
> > NAME.pot.in in the same directory as settings.xml).
> >
> > Christoph
> >
> >> Thanks
> >> Alireza
> >>> On Feb 8, 2016, at 10:28 AM, Christoph Junghans <[email protected]>
> wrote:
> >>>
> >>> 2016-02-07 19:50 GMT-07:00 Alireza Moradzadeh <[email protected]>:
> >>>> Dear Users,
> >>>>
> >>>> Let's suppose I have two molecules one composed of three beads and the
> >>>> second one composed of just one bead. mol1 : A B C & mol2 : D
> >>>> what's is the number of energy groups in gromacs.  should it be
> like:  mol1
> >>>> mol2  and energygrp_table: mol1 mol1 mol1 mol2 mol2 mol2 or A A A B A
> C A D
> >>>> B B B C B D C C C D
> >>> Yes, that is correct!
> >>>
> >>>>
> >>>> Thanks,
> >>>> Alireza
> >>>>
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> >>>
> >>>
> >>> --
> >>> Christoph Junghans
> >>> Web: http://www.compphys.de
> >>>
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> >
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