Hi,

I am not sure what exactly you are doing but it looks like you are 
trying to find a coarse-grained interaction potential for a dilute 
system (benzene in water). If this is the case, IBI is not the method to 
use. You can calculate the average force on your harmonic restraint as a 
function of r (restrain distance), integrate over r and subtract the 
entropic part (1/r scaling). You will then get your potential of mean 
force. If you have three equivalent beads, divide it by 3. You 
might need to do umbrella sampling to get good statistics for short separations.

Best,
Denis

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