-----BEGIN PGP SIGNED MESSAGE-----
Hash: SHA1
Hello Thenmalar--
> I have a question regarding the methyl RDC. This is in connection
> with the answer posted by Prof. Clore (dated Dec 11, 2003) as an
> answer to Dr. Andrew's Mail. It has been given in the answer that
> for methyl RDCs, for instance, in the case of Ala, we have to use
> CA-CB in the selection. It has also been mentioned in the paper by
> Ottiger et al ( JMR 365 1998) that the gyromagnetic ratio and bond
> distance for methyl group will be absorbed by xplor routine. I am
^^^^^^^^^^^^^^^^^^^^^^^^^
I'm not sure what is meant by this statement. See below.
> bit confused, because, if we use CA-CB for Ala methyl, how can we
> differentiate RDC from methyl group and that is from CA-CB itself.
If you only have CA-CB measurements, then the prefactor is absorbed in
the value of Da.
In Xplor-NIH's python interface, there are facilities to automatically
calculate the prefactors necessary when you want to use multiple
measurements made in a single medium. This facility will (currently) not
correctly handle CA-CB dipolar couplings, but you can easily get around
this manually. Please contact me if this is what you wish to do.
best regards--
Charles
-----BEGIN PGP SIGNATURE-----
Version: GnuPG v1.4.2.2 (GNU/Linux)
Comment: Processed by Mailcrypt 3.5.8 <http://mailcrypt.sourceforge.net/>
iD8DBQFETsnkPK2zrJwS/lYRAv6bAJ9mFtRFFOc/Ht0M+v22+0qMmJHU5gCfV8Ap
NzibKgXU179nB0GD1wvbe+A=
=JbeZ
-----END PGP SIGNATURE-----
