Hi, I'm testing different qualifiers and keys in GFF files, but I get strange error messages when I try to open them in Artemis (possibly because of my limited experience with the GFF format). The GFF file I made look like:
Test_0001 Glimmer3 CDS 421 1479 . + . transl_table=11;locus_tag=Test_0001;gene=dnaA;product=Chromosomal replication initiator protein dnaA Test_R0001 Glimmer3 tmRNA 1469 2047 . + . locus_tag=Test_R0001;gene=ssrA;product=RNA ssrA (tmRNA) Test_0002 Glimmer3 CDS 2081 3220 . + . transl_table=11;locus_tag=Test_0002;product=predicted ORF Test_R0002 Glimmer3 tRNA 3210 3929 . + . locus_tag=Test_R0002;product=tRNA-Ala Test_0003 Glimmer3 CDS 4310 4510 . - . transl_table=11;locus_tag=Test_0003;gene=swrA;product=pSwarming motility protein swrA Test_R0003 Glimmer3 rRNA 4532 4732 . + . locus_tag=Test_R0003;gene=rrf;product=5S ribosomal RNA And the error messages I get are: tmRNA is not a valid key tRNA can't have a locus_tag as qualifier rRNA can't have a gene as qualifier The later two warnings still seem to pass through, but it refuses to accept tmRNA as a valid key, which I don't understand? Regards, Stefanie Lager _______________________________________________ Artemis-users mailing list Artemis-users@sanger.ac.uk http://lists.sanger.ac.uk/mailman/listinfo/artemis-users
