Hi Stephanie

They are warnings and can be ignored in this case. These are controlled by

etc/qualifier_types_gff and etc/feature_keys_gff

I shall add in tmRNA and in feature_keys_gff and locus_tag.

Regards
Tim

On 3/5/10 1:38 PM, "Stefanie Lager" <stefaniela...@gmail.com> wrote:

> Hi,
> 
> I'm testing different qualifiers and keys in GFF files, but I get
> strange error messages when I try to open them in Artemis (possibly
> because of my limited experience with the GFF format). The GFF file I
> made look like:
> 
> Test_0001 Glimmer3 CDS 421 1479 . + . transl_table=11;locus_tag=Test_0001;gene
> =dnaA;product=Chromosomal
> replication initiator protein dnaA
> Test_R0001 Glimmer3 tmRNA 1469 2047 . + . locus_tag=Test_R0001;gene=ssrA;produ
> ct=RNA
> ssrA (tmRNA)
> Test_0002 Glimmer3 CDS 2081 3220 . + . transl_table=11;locus_tag=Test_0002;pro
> duct=predicted
> ORF
> Test_R0002 Glimmer3 tRNA 3210 3929 . + . locus_tag=Test_R0002;product=tRNA-Ala
> Test_0003 Glimmer3 CDS 4310 4510 . - . transl_table=11;locus_tag=Test_0003;gen
> e=swrA;product=pSwarming
> motility protein swrA
> Test_R0003 Glimmer3 rRNA 4532 4732 . + . locus_tag=Test_R0003;gene=rrf;product
> =5S
> ribosomal RNA
> 
> And the error messages I get are:
> 
> tmRNA is not a valid key
> tRNA can't have a locus_tag as qualifier
> rRNA can't have a gene as qualifier
> 
> The later two warnings still seem to pass through, but it refuses to
> accept tmRNA as a valid key, which I don't understand?
> 
> Regards,
> 
> Stefanie Lager
> 
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