Hi Tom, You can try the MEROPS database. There is a search feature called "What peptidases can cleave this bond".
http://merops.sanger.ac.uk/cgi-bin/specsearch.pl Cheers, Jack On Thu, Mar 31, 2011 at 6:59 AM, Brett, Thomas <tbr...@dom.wustl.edu> wrote: > Hi all: > I was wondering if anyone had any tips on identifying proteases. I have a > protein for which I know the proteolytic cleavage site. What are the best > ways to identify the protease that does the cutting either: > 1) bioinformatically (i.e., a good database to search using the cleavage > site or a consensus) > 2) experimentally (some engineered substrate to trap/identify the substrate > or any other method?) > Thanks in advance > -Tom > > Tom J. Brett, PhD > Assistant Professor of Medicine > Division of Pulmonary and Critical Care > Washington University School of Medicine > Campus Box 8052, 660 S. Euclid > Saint Louis, MO 63110 > http://brettlab.dom.wustl.edu/
