I think that BCA assay is what you are looking for. Le 13 févr. 2014 à 16:06, Raji Edayathumangalam <r...@brandeis.edu> a écrit :
> Dear CC4BBers, > > I am trying to figure out what is the best way to determine the protein > concentration of my membrane protein. My purified membrane protein is in 20mM > Tris pH 7, 150mM NaCl and 0.02% DDM (CMC of DDM=0.0076%). > > After reading the friendly manuals and searching online, I've learned that > detergents interferes with assays like Bradford but can't find good > descriptions of what works best. For now, I am trying to estimate > concentration from absorbance at 280nm and using molar extinction > coefficients based on aromatic amino acids, but again suspect detergent > interference. I would like to know what other folks working on membrane > proteins are doing. > > Thanks very much. > Raji > > -- > Raji Edayathumangalam > Instructor in Neurology, Harvard Medical School > Research Associate, Brigham and Women's Hospital > Visiting Research Scholar, Brandeis University > Xavier Brazzolotto, PhD Département de Toxicologie et Risque Chimique Institut de Recherche Biomédicale des Armées BP 73 91223 Brétigny sur Orge France Cell: +33 (0) 6 58 36 39 09 Actual Street Adress Institut de Biologie Structurale Equipe DYNAMOP 6 Rue Jules Horowitz 38027 Grenoble Cedex 1 France Phone: +33 (0) 4 57 42 87 19 The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
