Try using DNA as a search model - this has worked very successfully in our hands before.
Tony. - - - - - - - - - - - - - - - - - - Dr Antony W Oliver Senior Research Fellow CR-UK DNA Repair Enzymes Group Genome Damage and Stability Centre Science Park Road University of Sussex Falmer, Brighton, BN1 9RQ - - - - - - - - - - - - - - - - - - email: antony.oli...@sussex.ac.uk<mailto:antony.oli...@sussex.ac.uk> tel (office): +44 (0)1273 678349 tel (lab): +44 (0)1273 677512 http://www.sussex.ac.uk/lifesci/oliverlab http://tinyurl.com/aw-oliver - - - - - - - - - - - - - - - - - - On 24 Jul 2014, at 12:50, dusky dew <duskyde...@gmail.com<mailto:duskyde...@gmail.com>> wrote: DEAR ALL I am trying to solve the structure of a protein DNA complex with molecular replacement. The resolution in about 2.5 angstrom. The spacegroup is P21. The search model has about 50% identity and is a dimer of a dimer. The problem is the unit cell is huge and so the number of molecules in asu becomes 4 or 3. I am not finding any solution with phaser/molrep. Please suggest. Thanks Yang zhang
