Screen for heavy atoms with native gel. Run protein with variety of heavy atoms. If you see a discreet shift in the native gel, then you will get derivative. If you don't see a shift, don't bother soaking. It's worked for me every time. Also, ammonium sulfate will mess up your heavy atoms, so if that's in your condition, you need to substitute AmSulf with LiSO4 in your soak.
Nick On Wed, Nov 5, 2014 at 2:20 PM, Giulliana Rangel <giulliana.ran...@gmail.com > wrote: > Dear all, > > > I would like to known if someone could help me with some idea about the > phase problem. > > I am a beginner in crystallography and the first time I tryed to solve the > structure by Molrep, amore, mr. Bump and I didnt find anything for > molecular replacement. > > Thus, currently I've tried to soak heavy-atom ( Hg, Pt, I, Pb) and the > diffraction was good, the results in XDS didnt show so good. I didnt look > the heavy atom in density. > > I appreciate any suggestion and ideas what I could do. > > Best regards, > > > > -- > Giulliana Rangel > Mestranda PPG-Biotecnologia UNIFESP > Laboratório de Biologia Estrutural > Tel.: (12) 3309-9698 > Rua Talim 330, Vila Nair > CEP 12231-280 > São José dos Campos - SP > > -- [This e-mail message may contain privileged, confidential and/or proprietary information of H3 Biomedicine. If you believe that it has been sent to you in error, please contact the sender immediately and delete the message including any attachments, without copying, using, or distributing any of the information contained therein. This e-mail message should not be interpreted to include a digital or electronic signature that can be used to authenticate an agreement, contract or other legal document, nor to reflect an intention to be bound to any legally-binding agreement or contract.]
