Hi Giuliana,
if your crystal diffracts to 3 Å or better and you have at least one
Cys/Met per 25 residues, put it on the home source and collect until the
sulfur signal rises above the noise. Then solve by SAD.
http://www3.imperial.ac.uk/xraycrystallography/learning/sulfur_sad
Andreas
On 05/11/2014 7:20, Giulliana Rangel wrote:
Dear all,
I would like to known if someone could help me with some idea about the
phase problem.
I am a beginner in crystallography and the first time I tryed to solve
the structure by Molrep, amore, mr. Bump and I didnt find anything for
molecular replacement.
Thus, currently I've tried to soak heavy-atom ( Hg, Pt, I, Pb) and the
diffraction was good, the results in XDS didnt show so good. I didnt
look the heavy atom in density.
I appreciate any suggestion and ideas what I could do.
Best regards,
--
Giulliana Rangel
Mestranda PPG-Biotecnologia UNIFESP
Laboratório de Biologia Estrutural
Tel.: (12) 3309-9698
Rua Talim 330, Vila Nair
CEP 12231-280
São José dos Campos - SP
--
Andreas Förster
X-ray Crystallograhpy Facility Manager
Centre for Structural Biology
Imperial College London
